rabbit polyclonal abs against rhoa antibody  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: Obesogenic diet-induced gut barrier dysfunction and pathobiont expansion aggravate experimental colitis

doi: 10.1371/journal.pone.0187515

Figure Lengend Snippet: (A) RT-qPCR analysis of Occludin and Zo-1 using RNA from the distal ileum of normal chow diet (NCD)- or HFD-fed mice ( n = 5 each). (B) Representative immunohistochemistry of distal ileal sections using anti-Occludin or -Zo-1 mAb. (C and D) RhoA expression in the intestine of mice fed NCD or HFD. mRNA expression (C) and representative immunohistochemistry (D) of RhoA in the small intestine. (E) Serum levels of FITC 4 h after oral FITC-dextran administration to NCD- or HFD-fed mice by oral gavage ( n = 5 each). Original magnification ×200 for all images. Scale bar, 100 μm. All values are expressed as the means ± SEM. A student’s t -test was used to compare values between two groups. * P < 0.05, ** P < 0.01.

Article Snippet: For Occludin, ZO-1, and RhoA staining, sections were incubated with rabbit polyclonal Abs against Occludin (40–4700, Invitrogen), ZO-1 (40–2200, Invitrogen), and RhoA (PAB18218, Abnova), respectively.

Techniques: Quantitative RT-PCR, Immunohistochemistry, Expressing